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National Repository of Grey Literature 2 records found  Search took 0.02 seconds. 
The development of new isotope-coded cross-linkers for label free quantification of proteins
 Procházková, Valérie ; Kukačka, Zdeněk (advisor) ; Vaňková, Pavla (referee)
Elucidating the structure of proteins in living organisms is an important step in describing their role. One method of structural biology is chemical cross-linking in combination with mass spectrometry, which is a commonly used tool for characterisation of the tertiary structure of proteins or protein-protein interactions. This method is based on the reaction of a cross-linking agent consisting of two reactive groups linked by an arm of a defined length to form a covalent bond. The main aim of the project was to verify whether chemical cross-linking using istopically labeled cross-linking agents can be used for quantification of two different structural states. The reagents used in this work were DSPU and an isotopically labeled form of DSPUx. Both the DSPU and DSPUx reagents contain a labile urea molecule in their structure, which is cleaved into characteristic fragments during collision-induced dissociation, allowing the identification of cross-linked peptides. Using top-down and bottom-up approaches, it was found on selected proteins (insulin, human carbonic anhydrase, and bovine serum albumin) that DSPU and DSPUx reagents interact with selected peptides and proteins and are suitable for quantification of structural changes. Subsequently, structural differences in the presence (holoform) and...
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The development of new tyrosin binding cross-linkers for structural characterization of proteins
 Karpíšek, Michael ; Kukačka, Zdeněk (advisor) ; Talacko, Pavel (referee)
Proteins are cornerstones of all living organisms. A lot of energy is invested in studying structures of proteins, because their function is determined by their structure. One of the techniques used to access the structure of proteins is chemical cross-linking in combination with mass spectrometry. In spite of their number, most of the cross-linkers bind few aminoacids such as lysine, cysteine or glutamic and aspartic acid. In our work we tried to develop tyrosin-binding cross-linkers. Using top down and bottom up approaches we studied the influence of N-hydroxysuccinimide esters and imidazole on the reactivity of the cross-linker in different pH on model proteins. According to the acquired data there is a difference in overal reactivity and specifity between the used cross-linkers. The cross-linkers containing imidazole modified tyrosine more although the difference was small and binding of tyrosine was not selective. [IN CZECH]
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